Dear morphometricians,
I would greatly appreciate your advice on analyses of geometric morphometric data in relationship to geographic distribution and ecology. I’ve just started giving a look at the literature. These are my first impression and questions:

1. When relationships between size/shape and latitude/longitude (or ecological variables) are investigated, two main methods seem to be used: regression (multivariate multiple for shape) and partial least square (only for shape). The general aim seems to be the study of clinal variation and of the influence of ecological variables on morphology. In this context, I would tend to see latitude/longitude or ecological variables as independent variables and size or shape as the dependent ones. However, often a PLS analysis is performed which does not make a distinction between independent and dependent variables and looks for patterns of covariation between the two blocks of symmetric variables. Why is this statistical model used in a context where the relationship between variables does not seem to be symmetric? 2. A closely related but more general question. If I use a regression model, I assume that the independent variable is measured without error (or with an error negligible compared to the dependent variable). What shall I do if I want to use a regression model (because I am trying to predict Y using X) but the error in the independent variable is not negligible? 3. Getting back to the analysis of geographic data, is it appropriate to use latitude and longitude as variables in a regression or PLS model even if those variables are angles used to locate a point on a (more or less) spherical object? The space of the statistical models (standard parametric regression or PLS) is Euclidean while latitude and longitude are coordinates of point on a curved space. If I am correct, the use of latitude and longitude will introduce an error which can be very large when the analysis concerns geographic variation on a large scale (across a continent, for instance). Did I get it all wrong? 4. If what I said in the previous points makes any sense, is there a way for testing this error? May I do something similar (correlation between matrices of distances) to what is done for testing the tangent space approximation to the shape space in geometric morphometrics? 5. Last one. Some advice about software. Is there any program which computes matrices of geographic distances pairwise among all specimens in a dataset and does this by using latitude and longitude as variables but measuring the exact distance across the surface of the earth (i.e., taking the curvature into account)?

I thank you very much, in advance, for your help, and I apologize for asking questions which are probably trivial and only reflect my scant knowledge of the subject.
All the best

Andrea



Dr. Andrea Cardini
Hull York Medical School
The University of York, Heslington, York YO10 5DD, UK
&
The University of Hull,Cottingham Road, Hull HU6 7RX, UK
tel. 01904 321752
fax 01904 321696
E-mail: [EMAIL PROTECTED]
http://www.york.ac.uk/res/fme/people/andrea.htm
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