Hello Morphmet,
Glad I found you! I am working on a project investigating the
influence of biological and ecological factors on the deposition of
cementum (mineralized tissue surrounding the roots of teeth). I was
hoping to create an easy & quick method of analysis which wildlife
researchers could use to interpret individual and population history.

But…

My biggest concern is that I am not getting an accurate estimation of
cementum deposition.  My current approach is to obtain linear
measurements of cementum width from digital images captured from a
compound microscope; this quite literally only gives me “snapshots” of
the tooth. There is the added variation caused by the fact that not
all teeth are “readable” in the same locations and cementum deposition
around the surface of the root is not uniform. In some areas the lines
that distinguish between years of deposition become merged and
unidentifiable. I have tried to address these issues by only using
teeth that can be read in one general area and limiting my comparisons
to that region.  I am further limited to using teeth that were
originally processed and mounted for age determination (slides
containing 8 longitudinal sections of the tooth root). However, it
would be pretty safe to say that I am not limited by sample size…

If those of you more experienced in acquiring morphometric data from
such vagarious biological sources have any suggestions, directions, or
comments I would love to hear from you! More information about me and
my current research (including sample pictures of sectioned teeth!)
can be viewed at:
http://www.biology.ualberta.ca/faculty/andrew_derocher/index.php?Page=4171
or contact me: [EMAIL PROTECTED]

Sarah




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