Thanks Colin and Goran for your replies.  Colin, we've actually got
several thousand optic disc photos which I plan to do a trace of, but
thanks for the information anyway.

One other question;

I've read that you can break down Fourier co-efficients using principal
component analysis (and have actually been having a play with the
'Shape' software available on this site).  I understand that PCA is a
data reduction technique.  However, I don't come from a maths background
and I'm having trouble visualising what PCA actually gives you in the
break-down analysis of a shape.  For example, using this software, for a
sample of outlines

Eigenvalue    Proportion(%)    Cumulative(%)    > 1/96

    Prin1     3.554525E-004           53.9578           53.9578    *

    Prin2     2.099497E-004           31.8704           85.8282    *

    Prin3     6.445964E-005            9.7850           95.6132    *

Can someone explain (in lay terms) what the numbers actually mean (in my
situation of an optic disc)?  Are they vectors describing direction and
magnitude?  I can't seem to find any good resources online that explain
PCA without a considerable background maths knowledge basis (maybe there
is no simple way!)

Is PCA an accepted scientific data analysis technique?

Thank you.

Paul

[EMAIL PROTECTED]

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