-------- Original Message --------
Subject: PGLS troubles
Date: Sun, 24 Feb 2008 17:01:55 +0100
From: [EMAIL PROTECTED]
To: morphmet <[EMAIL PROTECTED]>

Dear morphometrician,
I am Carlo Meloro and I am conducting a research on macroevolutionary
integration in two portions of mammalian carnivore mandible (the
corpus mandibulae and the ascending ramus).
I am wondering if you could give me some suggestions on Partial Least
Square that I am using to validate the possible co-variation between
the two mandible portions.
I have several problems with the results obtained on the same landmark
dataset (separeted in two Blocks: Block 1 = shape variables after GPA
from 9 landmark configurations; Block 2 = shape variables after GPA
from 5 landmark configurations) independently on tpsPLS and Ntsys ver
2.20 N.
There are several discrepancies after the permutation test. Below I
report the output obtained for the same dataset of landmark data for
only 7 specimens. I suppose that there should be a problem of sample
size: do you know any problem associated to sample size in Partial
Least Square analysis?
I appreciate any help to understand this kind of results.
Thank you very much in advance,
Carlo Meloro



Below is the example with a small dataset of 7 specimens:
Results for 7 specimens (Block 1 = 9lnd, Block 2 = 5 lnd) with tpsPLs:
"
Number and percent of squared singular values >= observed
(Expressed as a proportion of the total.)

       Dim.  Observed  Count  Percent
          1  0.939037     58     5.80%
          2  0.049432    921    92.10%
          3  0.007188    876    87.60%
          4  0.003313    698    69.80%
          5  0.000905    549    54.90%

Number and percent of cumulative squared singular values >= observed
(Expressed as a proportion of the total.)

       Dim.  Observed  Count  Percent
          1  0.939037     58     5.80%
          2  0.988470    137    13.70%
          3  0.995657    309    30.90%
          4  0.998970    422    42.20%
          5  0.999876    367    36.70%

HERE THE CUMULATIVE PERCENTAGE DOES NOT APPROACH 100%

Number and percent of correlations >= observed

       Dim.  Observed Count  Percent
          1  0.780128   225    22.50%
          2  0.479620   873    87.30%
          3  0.770183   288    28.80%
          4  0.663578   563    56.30%
          5  0.615494   683    68.30%
          6  0.589321   840    84.00%
"

   From this report no correlation between Singular Axis is significant.


[Hide Quoted Text]


Same Results of permutation test from Ntsys:
"
(Note: counts include observed and small percentages imply "significance")

Number and percent of squared singular values >= observed
(Expressed as a proportion of the total.)

       Dim.  Observed  Count  Percent
          1  0.939037     56     5.60%
          2  0.049432    920    92.00%
          3  0.007188    837    83.70%
          4  0.000905    796    79.60%
          5  0.000124    905    90.50%

Number and percent of cumulative squared singular values >= observed
(Expressed as a proportion of the total.)

       Dim.  Observed  Count  Percent
          1  0.939037     56     5.60%
          2  0.988470    130    13.00%
          3  0.995657    279    27.90%
          4  0.996563    722    72.20%
          5  0.996687    801    80.10%

Number and percent of correlations >= observed:

       Dim.  Observed Count  Percent
          1  0.780128    51     5.10%
          2  0.479620    81     8.10%
          3  0.770183    12     1.20%
          4  0.615494    92     9.20%
          5  0.589321    62     6.20%
          6  0.663578    26     2.60%
   From this report the correlation obtained for Dimensions 3 and 6 are
significant. The first Dimension approaches significance at 0.05 level
(5.10 %).
Note that there is a large discrepancy in Number and percent of
correlations >= observed from tpsPLS and Ntsys output.



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