-------- Original Message --------
Subject: RE: Outlines in tpsDig
Date: Fri, 7 Nov 2008 09:19:20 -0800 (PST)
From: Sarah Degroot <[EMAIL PROTECTED]>
To: <[email protected]>
References: <[EMAIL PROTECTED]>

I have been able to "rig" a way to get a constant starting point for outlines in tspDig. The program finds the outline by searching to the left of wherever you click, and when it finds the second "edge," that's where the points start (details are in the help documentation). I rotated all of my images so that the one homologous point was on the left side. Before I click to find the outline, I first note the y-coordinate of the landmark position, then mouse to the right until the cursor is out of the image. When I click, the first edge seen is somewhere around the image, while the second edge is exactly on the landmark point. Since my sample shapes are rather complex, however, I still check the tps files to make sure the points are starting in the right place--sounds tedious but actually not very difficult since the x-coordinate is at its minimum on the homologous landmark. This all does take a little longer than if I were able to click exactly on the homologous landmark position or right next to it, but at least it is appreciably faster than placing 220 points by hand.

________________________________

From: Sarah Degroot
Sent: Thu 23-Oct-08 11:35 AM
To: [email protected]
Subject: Outlines in tpsDig


I am trying to make my tps files multitask-- I have black & white images and want to digitize outlines such that I can use the points in both EFA and a sliding landmark analysis. However, some of the specimens are fairly complex, such that I need at least 220 points to capture the outline adequately. I don't want to place 220 points by hand, especially since my preliminary analysis involves over 570 specimens. I tried drawing a curve with tpsdig, but that took 28 points (minimally), and it captured the outline poorly (even though I resampled the curve with more points). I'm also not eager to place even 28 points on each of 570+ specimens--there has to be a faster way.

It seems the easiest thing to do would be to use the outline tool in tpsdig, but this tool appears to be inconsistent about where it places the starting point. I click just to the right of where I want the starting point, but when I look at the tps file, the starting point is clearly somewhere else around the outline, and usually nowhere near where I clicked. This is the case for both tpsdig 2.09 and 1.40 (1.40 has given me a few other outline anomalies, too, such as negative coordinates).

I need the starting position of the x-y coordinates for the outline to be in the same place on all specimens because (1) I am not correcting for starting position in EFA because I do not want the outline rotated (they are all oriented the same way, but the long axis is not necessarily the same), and (2) I want one fixed "landmark" as a reference point for the sliding landmark analysis.

I'd like something similar to Perez et al.'s (2006; J. Anat. 208: 769-784) placement of semilandmarks around molars, although I'm not quite sure how they did it. I can't imagine they placed all 80 points by hand (p. 772).

Suggestions are appreciated.

Sarah

Graduate Student
Rancho Santa Ana Botanic Garden / Claremont Graduate University



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