----- Forwarded message from a.vanhete...@roehampton.ac.uk -----

     Date: Thu, 27 Sep 2012 04:03:46 -0400
      From: a.vanhete...@roehampton.ac.uk
      Reply-To: a.vanhete...@roehampton.ac.uk
      Subject: RE: PCA of landmark data on wings
      To: morphmet@morphometrics.org

Dear Jason

The mirror effect is just an artifact and does not mean anything. It is 
probably due to rounding of the data done by the programs you use. I often have 
mirrored axes when I perform the exact same analysis in different computer 
programs. It is nothing to worry about and it also does not effect the 
interpretation of your data. Specimens that are on opposite sides of you axis 
are very different from each other (with respect to the features representing 
that axis) and specimens that have very similar scores on that axis are very 
similar (again with respect to the features representing that axis). If the 
specimens are reversed on your PC axis, the features determining that PC axis 
(say, round vs elongated wings) will also be reversed, so your conclusions 
should be the same. 
I hope that helps. 

Best wishes,

Anneke van Heteren
________________________________________
From: morphmet_modera...@morphometrics.org 
[morphmet_modera...@morphometrics.org]
Sent: 26 September 2012 21:48
To: morphmet@morphometrics.org
Subject: PCA of landmark data on wings

----- Forwarded message from Jason Mottern -----

Date: Wed, 26 Sep 2012 12:50:47 -0400
From: Jason Mottern
Reply-To: Jason Mottern
Subject: PCA of landmark data on wings
To: morphmet@morphometrics.org

Hello,
I am a new list member, as well as a novice with respect to geometric 
morphometric analysis. I am doing a morphometric analysis of landmark data on 
wasp wings, and I'm doing both PCA and CVA. I am analyzing males and females 
separately, and there are three species. When I run all four analyses (CVA and 
PCA for each sex), the directions along the principal component axes are 
reversed for the female PCA analysis only. In other words, the signs are all 
reversed on the PC scores relative to the other three analyses, so its graph is 
"mirrored" compared to the other three. I am most intrigued as to why this 
reversal occurs between the female PCA and female CVA. These two analysis are 
based on the exact same set of partial warp scores, though, of course, 
subsequent calculations are different. I don't understand how the directions of 
the vectors are determined, and why they might differ between a PCA and CVA 
analysis of the same data. The programs I'm using for the analyses are PCAG!
 en and CVAGen (Sheets, 2002). I apologize if I'm not articulating the 
phenomenon very well, but, like I said, I'm very new to this stuff. Any 
thoughts would be greatly appreciated. 
Cheers,
Jason Mottern
jmott...@ucr.edu<http://mailto:jmott...@ucr.edu>

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