----- Forwarded message from a.vanhete...@roehampton.ac.uk ----- Date: Thu, 27 Sep 2012 04:03:46 -0400 From: a.vanhete...@roehampton.ac.uk Reply-To: a.vanhete...@roehampton.ac.uk Subject: RE: PCA of landmark data on wings To: morphmet@morphometrics.org
Dear Jason The mirror effect is just an artifact and does not mean anything. It is probably due to rounding of the data done by the programs you use. I often have mirrored axes when I perform the exact same analysis in different computer programs. It is nothing to worry about and it also does not effect the interpretation of your data. Specimens that are on opposite sides of you axis are very different from each other (with respect to the features representing that axis) and specimens that have very similar scores on that axis are very similar (again with respect to the features representing that axis). If the specimens are reversed on your PC axis, the features determining that PC axis (say, round vs elongated wings) will also be reversed, so your conclusions should be the same. I hope that helps. Best wishes, Anneke van Heteren ________________________________________ From: morphmet_modera...@morphometrics.org [morphmet_modera...@morphometrics.org] Sent: 26 September 2012 21:48 To: morphmet@morphometrics.org Subject: PCA of landmark data on wings ----- Forwarded message from Jason Mottern ----- Date: Wed, 26 Sep 2012 12:50:47 -0400 From: Jason Mottern Reply-To: Jason Mottern Subject: PCA of landmark data on wings To: morphmet@morphometrics.org Hello, I am a new list member, as well as a novice with respect to geometric morphometric analysis. I am doing a morphometric analysis of landmark data on wasp wings, and I'm doing both PCA and CVA. I am analyzing males and females separately, and there are three species. When I run all four analyses (CVA and PCA for each sex), the directions along the principal component axes are reversed for the female PCA analysis only. In other words, the signs are all reversed on the PC scores relative to the other three analyses, so its graph is "mirrored" compared to the other three. I am most intrigued as to why this reversal occurs between the female PCA and female CVA. These two analysis are based on the exact same set of partial warp scores, though, of course, subsequent calculations are different. I don't understand how the directions of the vectors are determined, and why they might differ between a PCA and CVA analysis of the same data. The programs I'm using for the analyses are PCAG! en and CVAGen (Sheets, 2002). I apologize if I'm not articulating the phenomenon very well, but, like I said, I'm very new to this stuff. Any thoughts would be greatly appreciated. Cheers, Jason Mottern jmott...@ucr.edu<http://mailto:jmott...@ucr.edu> ----- End forwarded message ----- Consider the environment. Please don't print this e-mail unless you really need to. This email and any attachments are confidential and intended solely for the addressee and may also be privileged or exempt from disclosure under applicable law. If you are not the addressee, or have received this e-mail in error, please notify the sender immediately, delete it from your system and do not copy, disclose or otherwise act upon any part of this email or its attachments. Internet communications are not guaranteed to be secure or virus-free. University of Roehampton does not accept responsibility for any loss arising from unauthorised access to, or interference with, any Internet communications by any third party, or from the transmission of any viruses. Any opinion or other information in this e-mail or its attachments that does not relate to the business of University of Roehampton is personal to the sender and is not given or endorsed by University of Roehampton. University of Roehampton is the trading name of Roehampton University, a company limited by guarantee incorporated in England under number 5161359. Registered Office: Grove House, Roehampton Lane, London SW15 5PJ. An exempt charity. ----- End forwarded message -----