----- Forwarded message from al cardini <alcard...@gmail.com> -----

Date: Wed, 31 Jul 2013 04:33:53 -0400
From: al cardini <alcard...@gmail.com>
Reply-To: al cardini <alcard...@gmail.com>
Subject: Re: MorphoJ and procrustes ANOVA
To: morphmet@morphometrics.org

They won't answer all your questions and I can't do more right now as I have meetings but you may find examples of tests for measurement error (and also group differences in one case) in these two papers:

Viscosi V., Cardini A., 2011 - Leaves, taxonomy and geometric morphometrics: a simplified protocol for beginners. PLoS ONE 6(10): e25630. doi:10.1371/journal.pone.0025630
http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0025630#s5

Franklin D., Cardini A., Flavel A., Kuliukas A., Marks M.K., Hart R., Oxnard C., O’Higgins P., 2013. Concordance of traditional osteometric and volume rendered MSCT interlandmark cranial measurements. International Journal of Legal Medicine, in press: DOI: 10.1007/s00414-012-0772-9.
https://docs.google.com/file/d/0BxZeFy3y3MEpLWdyazM2QTdCNG8/edit?usp=sharing

In both cases, you'll find also example datasets to replicate the analyses we did. Look for the suppl. online information or (more simply) check in my webpage:
https://sites.google.com/site/hymsfme/drandreacardini/publications

Good luck.
Cheers

Andrea


https://docs.google.com/file/d/0BxZeFy3y3MEpN3hnaTljUU5aZ00/edit?usp=sharing

On 31 July 2013 00:41, <morphmet_modera...@morphometrics.org> wrote:


----- Forwarded message from henry wallace <henry.ee.wall...@gmail.com> -----

Date: Tue, 30 Jul 2013 16:56:36 -0400
From: henry wallace <henry.ee.wall...@gmail.com>
Reply-To: henry wallace <henry.ee.wall...@gmail.com>
Subject: MorphoJ and procrustes ANOVA
To: morphmet@morphometrics.org

Hello to all,

My questions is about using the MorphoJ to implement the procrustes ANOVA.

I have need to find measurement errors in my data. This is step 1.  In the end, I need to discern a difference, or no difference, between two groups. This is step 2.

For step 1--finding measurement errors.

I have for each specimen 2 photographs digital. Each photograph was landmarked two times. This following the guidelines of MorphoJ User Manual page. Specimens are flat (fish) and is a 2 dimensional analyses.

With object symmetry selected, "Individuals" are the specimens, and "Error 1" is for image variation. "Error 2" is for landmarking variation. Specimens are fixed. Image and landmarkings are random. Any residual left over after effects are estimated is due to within group variations beyond what myself has introduced into the data. Is this correct in interpretation?


For  step 2-- finding differences.

According to Users Manual page,  I can select a second fixed main effect. This can be my grouping of specimens, no? This will tell me if groups are significantly differentiated above expectation given within group variation, no?

If yes, is the comparison "corrected" for random variations in image and landmarkings? I ask because Users Manual says that output contains random factor matrices, one of which has "The variance and covariance components...corrected for 'lower-level' effect." Is this correct in interpretation?

To clarify previous question, if I export this outputted individual matrix to another softwares, such as SPSS, may I use it to perform ANOVA to t-test for my two groups, without needing to worry about measurement error effects?

If the answer is no, I cannot interpret the individual matrix output from procrustes ANOVA this way, may I simply average my data within individuals and then perform t-test in SPSS or the like?

To clarify previous question, may I use MorphoJ Procrustes ANOVA to only measure the measurement errors in my own data, which I subsequently subject to averaging within specimen and finally a t-test for 2 groups in another software?

--Henry
Beloit College



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NO DOCX, XLSX AND PPTX, PLEASE!!!

COAUTHORS AND CONTRIBUTORS, PLEASE, FOR SHARING FILES, CONVERT THEM IN DOC, XLS (UNLESS LONGER THAN 256 COLUMNS) AND PPT


Dr. Andrea Cardini
Researcher in Animal Biology
Dipartimento di Scienze Chimiche e Geologiche, Università di Modena e Reggio Emilia, l.go S. Eufemia 19, 41121 Modena, Italy

Honorary Fellow
Centre for Anatomical and Human Sciences, Hull York Medical School
University of Hull, Cottingham Road, Hull, HU6 7RX, UK
University of York, Heslington, York YO10 5DD, UK

Adjunct Associate Professor
Centre for Forensic Science , The University of Western Australia
35 Stirling Highway, Crawley WA 6009, Australia

E-mail address: alcard...@gmail.com, andrea.card...@unimore.it, andrea.card...@hyms.ac.uk, andrea.card...@uwa.edu.au

Webpage: http://sites.google.com/site/hymsfme/drandreacardini
and: http://www.dscg.unimore.it/site/home/ricerca/aree-di-ricerca/evolution-taxonomy-and-forensics.html

Datasets: http://ads.ahds.ac.uk/catalogue/archive/cerco_lt_2007/overview.cfm#metadata
Editorial board for:
    Zoomorphology: http://www.springer.com/life+sciences/animal+sciences/journal/435
    Journal of Zoological Systematics and Evolutionary Research: http://www.wiley.com/bw/journal.asp?ref=0947-5745&site=1
    Hystrix, the Italian Journal of Mammalogy: http://www.italian-journal-of-mammalogy.it/





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