Hello Morphomet group,
I am trying to conduct comparisons of the structure of fluctuating 
asymmetry (FA) in squamate skulls in MorphoJ, both to FA structure in other 
taxa and to symmetric shape covariance within the same taxa. I am using 
matrix correlation with Mantel test for the comparisons. Normally, I 
believe it is correct to use the Ind*Side covariance matrix produced 
directly from Procrustes ANOVA for the matrix correlations. But I am also 
trying to do this with independent contrasts, which is where confusion 
starts.

To create a phylogenetically-independent covariance matrix of FA I have 
been mapping FA scores of shape data after Procrustes ANOVA onto a 
phylogeny and then constructing a covariance matrix from the independent 
contrasts. 

What confuses me is:
1. What FA scores are and is it meaningful to construct covariance matrices 
from them?
2. Why Morphoj gives options to construct covariance matrices using 
symmetric or asymmetric components from the independent contrasts of FA 
scores?
(I have tried both and found they are different)
3. Is the asymmetric component of shape (in MorphoJ) directional or 
fluctuating asymmetry? In one paper (Fig. 6), 
<http://www.jstor.org/stable/10.1086/650372> Klingenburg states it is the 
latter, whereas I always assumed it is the former

I hope this all makes some sense!

Best,

Alex Marshall
MSci Biology student
University College London
[email protected] 

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