I am examining relationship between morphometric data and climate variables with PLS analysis for 2 *Alnus* species (every species with 2 populations). I found significant correlation between these two blocks, and PLS1 explained 99.8% of covariation. On the plot of PLS1 and PLS2 for block 1, shape differences correspond to differences between species. But, I am also interested in shape differences in relation to climate between populations within species. I ran new PLS for every species separatly and also found significant correlations. In terms of interpretation, is it relevant to perform PLS for only two populations (when only PLS1 is calculated)? Best regards, Milena -- MORPHMET may be accessed via its webpage at http://www.morphometrics.org --- You received this message because you are subscribed to the Google Groups "MORPHMET" group. To unsubscribe from this group and stop receiving emails from it, send an email to morphmet+unsubscr...@morphometrics.org.