I am examining relationship between morphometric data and climate variables 
with PLS analysis for 2 *Alnus* species (every species with 2 populations). 
I found significant correlation between these two blocks, and PLS1 
explained 99.8% of covariation. On the plot of PLS1 and PLS2 for block 1, 
shape differences correspond to differences between species. But, I am also 
interested in shape differences in relation to climate between populations 
within species. I ran new PLS for every species separatly and also found 
significant correlations. In terms of interpretation, is it relevant to 
perform PLS for only two populations (when only PLS1 is calculated)? 

Best regards,


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