Hi, I've started a new project and came to the point of marking fixed and semi landmarks. Not all pictures are satisfying, mostly because of the posture of the larvae during photographing (sometimes raising it's tail). So in order to reduce the noise by the animal posture, I thought it would be helpful to separate head and tail as was done in Levis et. al. 2016, Biol. J. Linn. Soc. I'm using the landmarks 1, 20 and 48 as fixed landmarks, and all the rest are semi landmarks. I'm not sure of using 20 and 48 as fixed landmarks, and I wonder if I can use landmark 40 as fixed landmark since it is restricted by both x (side line) and y (dorsal connection of the tail fin). Can/Should I use the eye as a fixed landmark for the head (i.e. can it interfere with interpreting the head contour)? Here is an example to show what I mean: https://drive.google.com/file/d/1iO7lCN3ZCtV7DF9vsczkb_EYoSli1Orr/view?usp=sharing I'll be happy if you can advise on that. Thank you, Avi
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