Thanks to everyone who answered.

Dear Dr. Rohlf,
I assumed great variability would be a problem.
I was thinking the best way of digitizing would be to draw a curve on the 
outline of each leaf (simple and lobed leaves). Resample curves by length with 
approx. 40 landmarks. Then append curves to landmarks. Two landmarks, the leaf 
tip and base, would be fixed others could be defined as semi-landmarks. Is this 
Alternatively, we could analyse simple and lobed leaves separately.
Thank you a lot for your help.


From: f.james.rohlf []
Sent: Wednesday, March 14, 2018 3:59 AM
To: Tina Klenovšek
Subject: Re: [MORPHMET] digitizing very variable leaves

An assumption of the usual GMM methods is that shape variation is "small". I 
think these leaves exceed this quite a bit! Another problem is landmarks. How 
to match leaves with and without lobes?

F. James Rohlf, Distinguished Prof. Emeritus
Dept. Anthropology and Ecology & Evolution
Stonybrook University

-------- Original message --------
From: 'Tina Klenovšek' via MORPHMET <>
Date: 3/12/18 10:56 AM (GMT-10:00)
Subject: [MORPHMET] digitizing very variable leaves

Hello everyone,

we would like to digitize tree leaves that are very variable (from simple oval 
to strongly lobed on one tree).

1.)    I am wondering if TpsDig can do some kind of automatic digitizing like 
the LeafAnalyser software:, which evenly distributes 
a defined number of landmarks on the leaf outline...

LeafAnalyser does not seem flexible or precise enough. Or I can’t use it 
properly. Any experience?

2.)    Is it possible/sensible to put objects that are so differently shaped 
(photos attached) into the same group or is it better to analyse simple and 
lobed leaves separately?

I apologize if similar questions have been already answered...

Kind regards,


doc. dr. Tina Klenovšek, koordinatorica doktorskega
študijskega programa Ekološke znanosti
Univerza v Mariboru | University of Maribor
Fakulteta za naravoslovje in matematiko
Faculty of Natural Sciences and Mathematics
Koroška cesta 160, 2000 Maribor, Slovenija
T: +386 41 808 366

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