Dear Elena,

If all pictures are at the same scale, ok. However, I'd recommend you to
set scale factor (in tpsDig2: Options/Image tools/Measure).
Centroid size is commonly used as a proxy to the specimens sizes, but you
could use other measurements as well.
"all landmarks coordinates are based on the centroid" - it is the opposite:
the centroid is the mean coordinate of one specimen's LMs set. By using the
centroid size as independent variable one can explore the allometry in the

Hope it help.

best regards,
*Pedro S. R. Romano*
Professor Adjunto IV
Departamento de Biologia Animal <>
Universidade Federal de Vi├žosa <>
+55 31 38993775

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