Hi Mike,

Can you try using the latest CVS snapshot?
Unfortunately sourceforge seems to have silently deleted the cron job that updates the cvs snapshot and it hadn't been updated since May(!). That should be fixed now and the current snapshot is available from:
http://mpiblast.sf.net/mpiblast-latest.tar.gz

You will need to run
aclocal && autoheader && automake -a && autoconf
prior to running ./configure because the cvs snapshots don't include the complete build system.

Also, does using the -b and -v options to limit the number of results have any effect on whether the error appears?

-Aaron


Mike Muratet wrote:

Greetings

This question appears to have come up before on other lists, but I don't see an answer. I recall we've posted about this problem before but I don't think we ever got the problem resovled. I created two databases using the commands below from the Ensembl human and mouse
databases. I tried to run a query with:

setenv data_root /scratch/uahmam
setenv blast_root /opt/asn/apps/mpiBlast_1.3.0/latest/bin

setenv MPI_REQUEST_MAX 128000
setenv MPI_TYPE_MAX 128000

echo "blast_root is "$blast_root
echo "data_root is "$data_root
cd /scratch/uahmam

time /usr/bin/mpirun -np 8 $blast_root/mpiblast --remove -p blastn -d Homo_sapiens.NCBI35.nov.cdna.fa -i novel_v11.fa -W 9 -r 1 -q -1
-G 1 -E 3 -o novel_v_Hsen.blast

where my .ncbirc file is as below:

[mpiBLAST]
Shared=/scratch/uahmam
Local=/scratch/uahmam

[NCBI]
Data= /opt/asn/apps/ncbi_6.1/data

[BLAST]
BLASTDB=/scratch/uahmam
BLATMAT=/opt/asn/apps/ncbi_6.1/data


mpiformatdb --nfrags=6 -t enHs -i Homo_sapiens.NCBI35.nov.cdna.fa -p F -n enHs mpiformatdb --nfrags=6 -t enMm -i Mus_musculus.NCBIM34.nov.cdna.fa -p F -n enMm

I get nothing but a million+ lines of:

ObjMgrNextAvailEntityID failed with idx 2048

It fills up my disk quota and crashes so I get nothing else.

I have found this line in the source code, but I can't figure out what's going on from that.

mpiblast seems to work OK for other databases/queries.

Can anybody offer any suggestions?

Thanks

Mike



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