Hi all,

I'm more on the computer than biological side, so excuse my ignorance on
this, but you guys have been a big help in the past and hope someone can
answer my question.

I have mpiblast running well on my system with a web interface that has
the basic Blast template that comes with NCBI's wwwblast.  A user of
mine asked me if there was a way I can limit by entrez/organism as seen
on the NCBI version's web interface for Blast near the bottom of the
page:

http://www.ncbi.nlm.nih.gov/BLAST/Blast.cgi?CMD=Web&LAYOUT=TwoWindows&AU
TO_FORMAT=Semiauto&ALIGNMENTS=50&ALIGNMENT_VIEW=Pairwise&CLIENT=web&DATA
BASE=nr&DESCRIPTIONS=100&ENTREZ_QUERY=%28none%29&EXPECT=10&FILTER=L&FORM
AT_OBJECT=Alignment&FORMAT_TYPE=HTML&GENETIC_CODE=1&NCBI_GI=on&PAGE=Tran
slations&PROGRAM=tblastx&SERVICE=plain&SET_DEFAULTS.x=21&SET_DEFAULTS.y=
9&SHOW_OVERVIEW=on&UNGAPPED_ALIGNMENT=yes&END_OF_HTTPGET=Yes&SHOW_LINKOU
T=yes

Does mpiblast have a flag or some kind of option for me to do this?  Do
databases have to be formatted in a specific way to enable this?  Or
does NCBI just BLAST against everything, and then based on your limiting
run some kind of script or finalizing function to parse out the
appropriate stuff you want?  This would be the easy way, but efficiency
and speed would be the problem here because of the wasted computation
cycles on stuff the user doesn't need.  I'm guessing this is probably
not their approach.  Any ideas or advice would be helpful in
implementing this option, no matter how tedious or difficult.

Thank you very much,
Ray Chan
Plant Sciences BioComputing Center
Univ. of Calif, Davis



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