Hi for all,
I need some help about Parallel BLAST. I will bee happy if anyone help me.
I have worked with parallel BLAST using split database.
I don’t have problem to execute on part of database and statistics values
when use WUBLAST, because use DBRECMAX and DBRECMIN parameters and I execute
Blast like virtual split database, get just the piece of all database, and the
e-value get right.
But I really want do everything work in NCBI_BLAST. I know the solution of
mpiBLAST and the list of GI number file. But, these solutions aren’t so good.
The first because the source of BLAST have to change. And the second, because
require that you use GI numbers in the FASTA identifier.
So, my question is:
1) Somebody knows some else solution to run process blast on split
database, and not changes the e-value with relation to run whole database?
If not, the difference between e-value with whole database and part of database
(using the parameter –z and –Y of ncbi_blast) is very important?
Example, I processed one sequence with whole database and just part of
database, using parameter –z, the result was:
(evalue)NR
(evalue) NR/2 using –z
SeqQuery Seq1DB 4e-66
6e-66
SeqQuery Seq2DB 4e-38
5e-38
This difference is relevant?
Thanks,
Daniel Xavier – PUC – Rio de Janeiro - Brazil
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