>
> Hello,
>
> I was trying the different options of the MZmine 2.1 and I imported one
> .mzXML file with MS1 data. The acquisition was done at a ESI-TOF, using 2
> different energy modes. The problem I faced is that while in the raw data I
> can spot many molecules and in high intensities, when I use the Chromatogram
> builder and look at the peaklist table some of the masses disappear.
>
For the Chromatogram Builder I used the default options (noise
level:default) and applied no filtering.
>
> Here is a link to the .mzXML file that I used:
> http://rapidshare.com/files/428879210/140710-104.mzXML.zip
>
> For example there,at time point 3:20, one can see a very intense peak with
> m/z = 453. However, if you go to the peaklist created after the Chromatogram
> builder option, and find the same retention time, the peak is no longer
> there. I already tried to change the options of the Chromatogram builder but
> nothing changed.
>
> Could you maybe help me understand what I'm doing wrong?
>
> Thank you very much
>
> Regards
> Anastasia
>
>
>
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