Dear Developers, 1. RANSAC alignment: Dear Dr. Sandra, your recommendation for the RANSAC alignment worked little better with alignment for at least 50% of the peaks . However, still I am not getting correct alignment for all the peaks. Kindly explain about the parameter "threshold value". With the setting of 4 sec(0.07) min to 15 sec (0.25 min) (Pluskal et al 2010), I don't see many aligned peaks in the preview. By increasing the threshold value >1.0 min, I do see alignment of most of the peaks in preview but still single peak is represented more than 2 mines in different set of samples (ex 120.0389 at 30.12 in few samples and 120.039 at 29.89 min). Please suggest me some tips to set the "threshold value". 2. peak deconvolution (XCMS) Please suggest some tips on setting up wavelet scales. At present, I am using WAVELET SCALES OF 0.30 -5.0 with peak duration of 0.15-5.0 by looking into known peaks. However, I am getting lot of noisy peaks with low m/Z values (70.00-90.00) at the beginning and end of the chromatogram. With kind regards Raghavendra Gunnaiah Ph.D. scholar Department of Plant Science McGill Univerisity, Canada
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