Hi Felipe,

> I’m a Brazilian student who works with metabolomics, and I would like to ask 
> you some questions regarding the tool MZmine 2.9. 
> I read the manual section on Mzmine 2.9 "Adduct search" where it says that I 
> have to select the possible options "sodium", "potassium" ... etc., if the 
> data comes from a positive ionization mode of LC / MS.
> My First question is: Do I need to build a database of possible masses of 
> substances, adding them the isotopic masses of sodium, potassium, etc. 
> (Custom database search)? 

No, you do not need to use the Custom database search at all.

> 
> The second one is: Should I build another database of possible masses of 
> substances, this time subtracting from them the required mass in case the 
> analysis is performed in negative ionization mode?

There is no need to create separate lists for positive and negative modes. 

Please note that this module does _not_ search for ionization adducts, but 
instead it searches for neutral adducts that are attached to the main ion.

Best regards,

Tomas

===============================================
Tomas Pluskal
G0 Cell Unit, Okinawa Institute of Science and Technology Graduate University
1919-1 Tancha, Onna-son, Okinawa 904-0495, Japan
WWW: https://groups.oist.jp/g0
TEL: +81-98-966-8684
Fax: +81-98-966-2890


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