Hi Tang,
I am not familiar with "Precursor ion mode". Can you send a sample data file?
Best regards,
Tomas
________________________________
From: 企研組-唐川禾 [cht...@nmmba.gov.tw]
Sent: Monday, April 01, 2013 2:20 AM
To: mzmine-devel@lists.sourceforge.net
Subject: [Mzmine-devel] How to make peak list from a data file of Precursor Ion
Scan
Hi,
I am trying to use MZmine 2.10 (no modify; OS: windows XP) that was downloaded
from the webset of MZmine 2 home page for my MS data processing. These data
(.wiff) were derived from ABI MS system using Precursor Ion Scan mode. The
original data (.wiff) were translated to the format of mzData file. I use the
mzData file for data processing in MZmine 2.10. I had tried two modules:
1) Raw data methods > Mass detection (Mass detector: Centroid; MS level: 2;
Mass list name: masses1) > Chromatogram builder (Mass list: masses1; Min time
span (min): 0.1; Min height: 100; m/z tolerance: 0.2; Suffix: chromatograms)
2) Raw data methods > MS/MS peaklist builder (m/z window: 0.1; Time window:
0.1; MS level: 2)
Both could not make the showing of peak list (as shown in attachment).
However, the 2D and 3D chromatograms can be displayed. Differently, another MS
data that was acquired by Q1 Full Scan mode could be processed using the module
as below:
Raw data methods > Mass detection (Mass detector: Centroid; MS level: 1; Mass
list name: masses2) > Chromatogram builder (Mass list: masses2; Min time span
(min): 0.1; Min height: 100; m/z tolerance: 0.2; Suffix: chromatograms)
Why it couldn't be operated for data file derived from Precursor Ion Scan mode.
If anybody can please make me the points in the problem I met? Thank you for
your time!
Have a nice day
Tang, Chuan-Ho
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