Here's a handy online tool for determining PCB-trace separation:  
http://www.smps.us/pcbtracespacing.html
There are 3 spacings noted (external, internal, and coated). I would 
recommend using 'external' wherever possible because it's the most 
conservative. Unseen flaws in PCB manufacturing happen, so using 
absolute-minimum spacing with high-voltage traces is a gamble.

The website mentions UL experiments that found PCB's can tolerate 40 
volts/mil. One mm is about 40mils.
I dont advise pushing PCB designs anywhere near that value, though.

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