I wouldn't bother filling the tires with nitrogen.  They're already filled 
with regular air, which contains about 78% nitrogen.
Yes, I think you have a leak, and since you're riding a bike that has 
spoked wheels (a 2008 Nighthawk 250, right?) I recommend you install a 
fresh innertube and after finding and removing the offending object that 
caused the leak.

On spoked wheels, the rims often have sharp bits that press against the 
innertube.  It's the reason you're supposed to install a new innertube 
every time you install a new tire.  Look for those sharp points and file 
them down while you have the tire off.  

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