App on a 2003 IIS server that uses the IIS integrated authentication. The app 
does not do the authentication, the IIS website does. Authentication is back to 
2008 R2 DC's. And I think that is the issue, this seems to have just started 
when I updated the DC's from 2008 to 2008 R2.

It is very odd, some users can hit it with integrated authentication and others 
cannot.  For a couple of days at a time those that cannot sometimes can. When 
the users fail I can see it in the security log, that the user is being checked 
against the local server account list...rather than the domain. If the user 
hits the popup with domain/user at that point it authenticates them just fine.

Authentication methods on the website appear correct, and have not been changed 
since the app was setup.  Anonymous is allowed and the IUSR account is valid 
and synced up correctly. Then only Integrated Windows Auth is checked.

~ Finally, powerful endpoint security that ISN'T a resource hog! ~
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