Dick Thomson wrote: " ..in some cases, the fungi becomes fluffy on the surface of the media in the flask and later starts to consume the orchid seedlings." ... "One possible cause is that there was more than one fungi in the peleton that was isolated from the plant and a non desirable fungi has now become dominant."
That is most likely the case. It is not easy to make a mono-fungal culture in the first place, and it requires some skill and routine to keep the subcultures free from contamination with unwanted fungi, bacteria, mites and other pests. And even then there will always be som losses. B} the way, the word "mycorrhiza" refers to a root or other plant part AND a symbiotic fungus. In other words, the phytobmont as well as the mycbiont. The isolated endophytic fungus is just "the fungus". Good luck with your cultures, Finn Rasmussen, Copenhagen. _______________________________________________ the OrchidGuide Digest (OGD) [EMAIL PROTECTED] http://lists.orchidguide.com/mailman/listinfo/orchids

