K Barrett, I was unable to view the abstract with the link you provided. Would it be possible for you to give me the reference? Or copy/paste?
>From what I know about paph cloning, there has been success with using in vitro plant tissue (see Chen et al 2003 and 2004). However, this method is impractical for as you may be well aware, paph seedlings are highly diverse. So far, my work in this area has found contamination to be a slight hurdle. I think I found a method to obtain sterile cultures but am I killing too much plant cells in the process? I need more time to find out. The biggest hurdle I am experiencing is locating in vivo plant tissue that is able to dedifferentiate. On a side note, it is still unknown how genetically identical micropropogation clones (of paphs) are from each other. In theory, all plants should be identical, but when you are take highly differentiated cells, dedifferentiate, and then change them to something else, mutations are bound to occur. In case you are wondering, I am studying paph micropropagation for my thesis. -Will
_______________________________________________ the OrchidGuide Digest (OGD) [EMAIL PROTECTED] http://lists.orchidguide.com/mailman/listinfo/orchids
