"K Barrett" <[EMAIL PROTECTED]> spaketh thusly: > Anyone have any information on how oryzalin acts to induce polyploidy? > AFAIK its an herbicide.
Oryzalin (Surflan) is a dinitroaniline herbicide with very low toxicity (LD50 >5,000 mg/kg in rodents, >1000 mg/kg in dogs), a negative Ames test, but a possible carcinogen. It is used as a selective, pre-emergence herbicide for grasses and weeds. It inhibits spindle formation in the meristem at low concentrations, and in the field (i.e., at concentrations consistent with the rate of application for which it is labeled) this will serve to block cell division, killing the meristem. It has been used to replace the rather nastier colchicine which, if my understanding is correct, also inhibits spindle fiber formation. Unsurprisingly, it is not labeled for use in ploidy modification. However, it has been used as such. http://www.liliumbreeding.nl/tulipa/oryza_taka.pdf The herbicide trifluralin (Treflan) has been used as well. Also a dinitroaniline herbicide, trifluralin has a similar toxicity profile, although there are some better data to support it being a carcinogen. Cheers, -AJHicks Chandler, AZ _______________________________________________ the OrchidGuide Digest (OGD) [email protected] http://orchidguide.com/mailman/listinfo/orchids_orchidguide.com
