Peter O'Byrne wrote:
Yukawa had no reason to suspect that his D. suzukii was a hybrid, so
he has not discussed this possibilty in his analysis. I showed his
paper to researchers doing similar work at the Molecular Biology lab
at Singapore University, and was told that you'd get identical results
if "D. suzukii" was a hybrid.
I don't agree. An F1 primary bybrid should have ribosomal RNA genes from
both parents. Amplifying total DNA, as Yukawa did, will amplify the genes
from both species simultaneously. Then, the results will depend on the
specific protocol followed (which is not specified in the materials and
methods). If Yukawa directly sequenced the PCR products, he should see
ambiguous nucleotides, usually designated "N," wherever the two parent
species differ. The results of sequencing a mixed PCR product are usually
quite obvious. If he cloned the PCR products and sequenced individual
clones, then a primary hybrid would have given him sequence identical to one
or the other parent, depending on which clone he sequenced. I can't see any
way that a primary hybrid could give clean sequence results that are
intermediate between the two parents.
Nick
--
Nicholas Plummer
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