Keith,
I have performed and developed ELISAs in my line of work as a Research Assistant in Molecular Biology and Immunology. Each assay, done in panels, requires a row, or serial dilution, of standards to be performed with it. The dilutions should then match up with results in a stepwise fashion when plotted on a graph (standard curve). Test sample results are then plotted along that line to give the amount of agent being searched for present. Getting lazy and using one standard curve for several tests (on different assay plates) assures that plate to plate variation will give inaccurate results, especially if done on different days with different reagent stocks used. Ways in which samples are prepared for assays can also obscure results and all samples should be done in at least duplicate, preferably triplicate or quadruplicate to help screen out false positives, but this is not assured.
Bonaventure
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Message: 4
Date: Wed, 30 Aug 2006 14:56:50 +0000
From: "K Barrett" <[EMAIL PROTECTED]>
Subject: Re: [OGD] viruses again
To: [email protected]
Message-ID: <[EMAIL PROTECTED]>
Content-Type: text/plain; charset="iso-8859-1"
Thanks for doing that Cynthia.
Open question: Does anyone have any other experience with the ELISA test (even in another line of work) so we can judge as to how correct it is? Like the rates of false negatives/false positives?
K Barrett
N Calif, USA
Message: 4
Date: Wed, 30 Aug 2006 14:56:50 +0000
From: "K Barrett" <[EMAIL PROTECTED]>
Subject: Re: [OGD] viruses again
To: [email protected]
Message-ID: <[EMAIL PROTECTED]>
Content-Type: text/plain; charset="iso-8859-1"
Thanks for doing that Cynthia.
Open question: Does anyone have any other experience with the ELISA test (even in another line of work) so we can judge as to how correct it is? Like the rates of false negatives/false positives?
K Barrett
N Calif, USA
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