Quote: >Now... Is it too insane for me to ask for a dumbed down version as to how >one does this? It seems awfully complicated. One would have to isolate the >gene/chromosome etc. Or does one just surmise its isolation from the >outcome of the cross?
With some difficulty: you might care to look at the Brassia napus paper that i referenced in the post. In essence, you need to cause a gamete (a pollen cell) to duplicate its genetic material, which you can do with colchicine and an electric shock, amongst other stimuli. The resulting 'fake diploid' has to be nurded (a typo which I am going to let stand as appropriate - I mean to type 'nursed') to adulthood through the usual mericlone process. Of course, you have to do this in huge parallel to get the desired combination of genes, and unless you have a whole mol biol lab (and know which genes you want) you have to grow them all to adulthood to get the one that you want. ______________________________ Oliver Sparrow +44 (0)20 7736 9716 www.chforum.org _______________________________________________ the OrchidGuide Digest (OGD) [email protected] http://orchidguide.com/mailman/listinfo/orchids_orchidguide.com
