Quote: Mike Mazur 

>I have been using a product containing harpin proteins that has been a 
>tonic for all orchids in my hobbyist's collection of 600 - 700 plants.

Harpins are derived from bacteria which attack plants, Erwinia, for example.
They are the proteins which the bacteria uses to punch through the cell wall
when mounting its attack. Plant cells have learned to recognise these and
react quickly to their presence, which they do through what is called
apoptosis, programmed cell death. 

Multicellular organisms have many strategies for managing invaders. Apoptosis
is one of these. Cells that are infected or damaged are identified or
self-identify, and this triggers a series of events which lead to cell death. 

Plant cells mostly keep apoptosis in house, as an unassisted suicide, so to
speak. There are, however, various pervasive signals such as jasmonic acid,
ethylene and, apparently, micro-RNAs which indicate the general presence of
pathogens. All of these contribute to reducing the threshold at which the
'hypersensitive response' occurs, making the plant pro-active in dealing with
bacterial invasion. Harpins trigger an immediate and local response, as
discussed, but they are also reported to cause the affected cells to issue
these more pervasive signals, preparing others in the plant to give their
lives for the greater good. 

Whether it is a good thing to maintain a crop in such a state of nervous
excitement is not really established. The harpins have been most studied in
the Solanaceae - notably, tomatoes and tobacco - where there is good evidence
that a pretreatment with harpins does help the plant to resist subsequent
challenge with e.g. Pseudomonas. A gene (pflp) from one of these, the sweet
pepper, accentuates the harpin hypersensitive response. It has been
engineering into Oncidiums (and into rice, bananas, broccoli) to increase
their resistance to infection. 

Earlier, I mentioned that soil contains an enormous number of micro-organisms,
and that plants were under permanent attack from these. One person writing in
response to this said that one should think of the poor human. We are an
assemblage of around one hundred trillion cells. Only ten percent of these are
human, and the rest are bacteria, fungi and so on that hitch a ride on us. 
______________________________

Oliver Sparrow
+44 (0)1628 823187
www.chforum.org

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