I've used the *ist-D (a pretty similar DSLR) to take high magnification 
photos of snow crystals. Not through a microscope - using a reverse 
mounted 50mm lens and bellows - but a fair degree of magnification.

I'd assume that you could focus by looking through the finder. the snow 
crystals are ephemeral (they sublime fairly quickly) but I still have 
time to take a look at the photo on the screen and make adjustments as 
needed. I think the *ist-D is a lot slower than the K100D, so you should 
be able to check and re-shoot if needed.

- MCC

PS: Snow crystals are here:

http://www.markcassino.com/galleries/snowflakes/flake_thumbs.htm

here

http://www.markcassino.com/galleries/stream/stream05.htm

And a few other places in the site...

- MCC

Paul wrote:
> Has anyone on this list attempted to use the 
> Pentax K100D to take microsphotographs using 
> a microscope? 
> 
> Given the lack of a Live View, is there anyway 
> a person can determine when the microscope image 
> is focused in the camera?
> 
> Yours,
> 
> Paul
> Louisiana
> 
> 
> 
>  
> ____________________________________________________________________________________
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-- 
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Mark Cassino Photography
Kalamazoo, Michigan
www.markcassino.com
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