This is a message from the Museumpests List. To post to this list send it as an email to [email protected] To unsubscribe please look at the footer of this email. ----------------------------------------------------------- It would be worth getting a copy of the article Effects of Fumigants and non-chemical treatments on DNA molecules and proteins: case studies on natural history specimens and proteinaceous components of museum objects by Rika Kigawa and Tom Strang published in the Pest Odyssey 2011 post-prints
http://www.pestodyssey.org/publication.php Here is the abstract "It has been shown that some fumigants such as methyl bromide, ethylene oxide, propylene oxide and methyl iodide can affect specimen DNA and amplifying DNA fragments from the extracted specimen DNA by PCR (polymerase chain reaction). On the other hand, sulfuryl fluoride and non-chemical meth-ods did not have any effect on DNA. After overview of the knowledge in 10 years, we report more detail on the effects on DNA by thermal methods (heating and low temperature) with samples precon-ditioned to various relative humidity (RH) values. Consequently, DNA of specimens pre-equilibrated with low or moderate RH remained intact after thermal treatments. But DNA of the samples pre-equilibrated with high RH or in extreme water-soaked condition was occasionally affected. Also we briefly overview our recent work on effects of pest controlling treatments on protein-based natural specimens." Rachael Perkins Arenstein A.M. Art Conservation, LLC Conservation Treatment, Preservation Consulting & Collection Management <http://www.amartconservation.com> www.amartconservation.com <mailto:[email protected]> [email protected] 917-796-1764 From: [email protected] [mailto:[email protected]] On Behalf Of Louis Sorkin Sent: Wednesday, October 10, 2012 9:09 AM To: [email protected] Subject: Re: [pestlist] heat treatment effect on DNA This is a message from the Museumpests List. To post to this list send it as an email to [email protected] To unsubscribe please look at the footer of this email. ----------------------------------------------------------- Actually it's very possible that it's degraded the DNA for molecular study investigations. Sent from my Verizon Wireless 4G LTE Smartphone [email protected] wrote: This is a message from the Museumpests List. To post to this list send it as an email to [email protected] To unsubscribe please look at the footer of this email. ----------------------------------------------------------- Elin - Heat treatment for stored product pests in grain silos, food plants, and certain kinds of warehouses has been practiced on a large scale for decades. The temperature needed to kill all stages of any insect is 130 degrees F. for 3 hours. We're not talking about temperature ranges which would affect DNA. Certainly large scale heat treatment of grain silos has not affected the grain's germination rates or its DNA. Tom Parker -----Original Message----- From: Elin Ohlsson <[email protected]> To: pestlist <[email protected]> Sent: Wed, Oct 10, 2012 6:05 am Subject: [pestlist] heat treatment effect on DNA This is a message from the Museumpests List. To post to this list send it as an email to [email protected] To unsubscribe please look at the footer of this email. ----------------------------------------------------------- We are having trouble with pest infestation in our herbarium and are considering heat treating instead of fumigation with toxic gases. (We are already working with cleaning the rooms, freezing and bagging of specimens but it is not enough). Is there anyone with experience of heat treatment in large areas or maybe has any documentation? We are mostly concerned about the effect on DNA. Thanks for any information Best regards, Elin Description: Beskrivning: Beskrivning: Description: Description: Description: Description: nrm_logo Elin Ohlsson Senior assistant Department of Cryptogamic botany 08-519 541 52 [email protected] Swedish Museum of Natural History P. O. Box 50007 SE- 104 05 Stockholm, Sweden www.nrm.se <http://www.nrm.se/> Krypto-S: <http://www.nrm.se/botany/krypto-s> http://www.nrm.se/botany/krypto-s 1 310 000 botanical specimens searchable through internet ---------------------------------------------------------------------- To send an email to the list, send your msg to [email protected] To unsubscribe from this list send an email to [email protected] and in the subject put: "unsubscribe" - no quotes please. You are receiving the Pestlist emails in standard mode. To change to the DIGEST mode send an email to [email protected] with this command in the body: set mode digest pestlist Any problems email [email protected] or [email protected] ---------------------------------------------------------------------- To send an email to the list, send your msg to [email protected] To unsubscribe from this list send an email to [email protected] and in the subject put: "unsubscribe" - no quotes please. You are receiving the Pestlist emails in standard mode. To change to the DIGEST mode send an email to [email protected] with this command in the body: set mode digest pestlist Any problems email [email protected] or [email protected] ---------------------------------------------------------------------- To send an email to the list, send your msg to [email protected] To unsubscribe from this list send an email to [email protected] and in the subject put: "unsubscribe" - no quotes please. You are receiving the Pestlist emails in standard mode. To change to the DIGEST mode send an email to [email protected] with this command in the body: set mode digest pestlist Any problems email [email protected] or [email protected] ---------------------------------------------------------------------- To send an email to the list, send your msg to [email protected] To unsubscribe from this list send an email to [email protected] and in the subject put: "unsubscribe" - no quotes please. You are receiving the Pestlist emails in standard mode. To change to the DIGEST mode send an email to [email protected] with this command in the body: set mode digest pestlist Any problems email [email protected] or [email protected]
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