So I'm having what I think is a similar issue with some local data.
We have the alignment + genomes from Bio.MSA.UCSC.dm3_multiz15way
built locally.  When I set my WORLDBASEPATH to only look locally, I
try the same code:



In [3]: msa = worldbase.Bio.MSA.UCSC.dm3_multiz15way()
In [4]: triCas2 = worldbase.Bio.Seq.Genome.TRICA.triCas2()
In [5]: seq = triCas2['singleUn_263'][2437:2453]
In [6]: msa[seq]
---------------------------------------------------------------------------
IOError                                   Traceback (most recent call
last)

/home/baldig/projects/genomics/nonsvn/output/fly/motifsearch/
2009_7_30_13_19_39_3_211_1/<ipython console> in <module>()

/home/dock/shared_libraries/lx64/pkgs/pythonsandbox/2.6.2/lib/
python2.6/site-packages/pygr-0.8.0-py2.6-linux-x86_64.egg/pygr/
cnestedlist.so in pygr.cnestedlist.NLMSA.__getitem__()

/home/dock/shared_libraries/lx64/pkgs/pythonsandbox/2.6.2/lib/
python2.6/site-packages/pygr-0.8.0-py2.6-linux-x86_64.egg/pygr/
cnestedlist.so in pygr.cnestedlist.NLMSASlice.__cinit__()

/home/dock/shared_libraries/lx64/pkgs/pythonsandbox/2.6.2/lib/
python2.6/site-packages/pygr-0.8.0-py2.6-linux-x86_64.egg/pygr/
cnestedlist.so in pygr.cnestedlist.IntervalFileDBIterator.loadAll()

/home/dock/shared_libraries/lx64/pkgs/pythonsandbox/2.6.2/lib/
python2.6/site-packages/pygr-0.8.0-py2.6-linux-x86_64.egg/pygr/
cnestedlist.so in pygr.cnestedlist.IntervalFileDBIterator.nextBlock()

IOError: Iterator has no database!  Please provide a db argument.



How long ago was the bug squashed?  I thought we were running on a
fairly recent version of pygr...  Would it be enough to update to the
latest version of pygr or should I rebuild the resources?

Thanks again!

On Aug 4, 7:07 pm, jbiesinger <[email protected]> wrote:
> fixedforme.  Thanks a ton for the quick response!
>
> On Aug 4, 6:25 pm, Namshin Kim <[email protected]> wrote:
>
> > Hi,
> > I had updated pygr using latest version as of today and restarted the XMLRPC
> > server. Please check it again.
>
> > Yours,
> > Namshin Kim
>
> > On Wed, Aug 5, 2009 at 10:19 AM, Christopher Lee <[email protected]> wrote:
>
> > > Hi,
> > > it turns out the XMLRPC server is running an old version of Pygr that
> > > had a bug in reloading of NLMSAs.  That was fixed about two months
> > > ago, but the server is running an older version.  We will restart the
> > > XMLRPC server with the 0.8 beta version.
>
> > > Thanks for reporting this!!!
>
> > > -- Chris
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