> 1. Is there a simple way in pymol to replace an amino acid in > a given structure? If so, how can you then check (other than > visibly) if there are any glaring steric/electrostatic clashes?
Yes, using the mutagenesis wizard. But no, there's no refinement/validation possible so it isn't as useful as it otherwise might be. > 2. Is there a way of incorporating non-native or modified > amino acids into the pymol builder function? Specifically > I'm thinking about fluorescently labeled amino acids, but I'd > assume the method is the same for any non-native amino acid. There is no prescribed way of doing this yet. Cheers, Warren
