To add to what Cameron said: * Cameron Mura <cm...@ucsd.edu> [2005-03-24 09:44] wrote: > Hi Hyun-Chul, > > It's easy to use PyMOL to build protein fragments in standard geometries > ("Build" menu in the Tcl/Tk GUI window), but I'm not sure about DNA. > Three non-PyMOL methods that I'm aware of are: > > (1) The program "B" (aka "Biomer") from Dave Case's group at Scripps > (http://www.scripps.edu/mb/case/Biomer/). It's a Java-based online > biopolymer-building package, and is freely accessible. > > (2) The utility "nucgen" that comes with the Amber software package is > useful for generating single- or double-stranded DNA and RNA structures > in a variety of geometries (see pg. 236 of the Amber v8 manual -- > http://amber.scripps.edu/doc8/amber8.pdf). Unlike "B", this runs from a > unix shell given plaintext input files, so would be more useful for > scripting and things like that. However, Amber is not free. > > (3) There used to be a web-based server known as "RBSCM: Rapid Building > of Standard 3D-Conformations of DNA/Protein" available at > "http://www.bioinf.co.uk/bsps/bsps_N.html", and I found it very useful > for quickly building standard geometries. The www.bioinf.co.uk site > doesn't seem to exist anymore (no redirects), but maybe you can track it > down elsewhere... > > There are probably other ways to go about this too (e.g., InsightII > biopolymer builder), but I think they're all > proprietary/closed-source/not-free.
There is an easy to use open-source program called NAMOT (http://namot.lanl.gov/) that allows one to type in a sequence and create a DNA molecule with options for single- or double-stranded and A, B or Z conformations. Cheers, Rob -- Robert L. Campbell, Ph.D. <r...@post.queensu.ca> Senior Research Associate phone: 613-533-6821 Dept. of Biochemistry, Queen's University, fax: 613-533-2497 Kingston, ON K7L 3N6 Canada http://adelie.biochem.queensu.ca/~rlc PGP Fingerprint: 9B49 3D3F A489 05DC B35C 8E33 F238 A8F5 F635 C0E2