Hi, I want to show an electrostatic surface of my protein for publication. In Pymol, I believe I can use generate-vacuum electrostatics. However, I am not quite sure what the difference is between absolute and relative protein surface potential. Could you help me with this? Second, I have a question about the slider that appears after the surface is calculated. What do the numbers represent, and what is changing as you slide to the left and right. I want to use the figure for publication and make sure I understand how to explain it properly.
Thanks, Shawn-