Orla, This is simply, for the most part. Here is what I do.
Load your *.pdb file. Then under WIZARD in the menu bar, select MUTAGENESIS. Then pretty much, follow directions. In the PyMOL VIEWER window, it will ask you to ' pick a residue.' Once you pick a residue, select the type of residue you want to mutate to by left clicking on [No Mutation] button and holding down (under Mutagenesis). This brings up a list of residues that you can then select from. Once you select the residue you want, hit APPLY, then DONE. If everything was done correctly, you should see your mutated residue. There are probably other ways to do this, but I hope this helps. If you would like screenshots of the steps, just let me know. Cheers, Nick ----- Original Message ----- From: Orla O'Sullivan To: pymol-users@lists.sourceforge.net Sent: Tuesday, April 18, 2006 11:31 AM Subject: [PyMOL] mutations Hi all I'm a newbie to Pymol and so far have been very impressed. However I have tried to mutate some residues in my structure and have hit a brick wall. Has anyone done this with pymol and let me know how to do it? Regards Orla Dr.Orla O'Sullivan Research Officer Biotechnology Moorepark Food Research Centre Teagasc Moorepark Fermoy, Co. Cork Ireland Tel: +353 - (0)25 - 42344
