Hi,
Just pure curiosity:
may I ask why you want to do spline interplation on fluorescence intensity as
function of concentration?
Particularly as it looks quite typical for an unknown problem's calibration
plot?
Claudia
On 02/05/2011 03:29 PM, Asan Ramzan wrote:
Hello R-help
I have the following data for a standard curve
concentration(nM),fluorescence
0,48.34
2,58.69
5,70.83
10,94.73
20,190.8
50,436.0
100,�957.9
�
(1)Is there function in R�to plot a spline.
(2)How can I interpolation,say 1000 point from 0nM-100nM and store this as a
data frame of concentration,fluorescence
(3)How can I modify�the code below so that instead of retrieving a concentration
with the exact value of fluorescence, it gives me concentration for the value
that is closest to that fluorescence.
�
subset(df,fluorescence==200.3456,select=concentration)
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Claudia Beleites
Dipartimento dei Materiali e delle Risorse Naturali
Università degli Studi di Trieste
Via Alfonso Valerio 6/a
I-34127 Trieste
phone: +39 0 40 5 58-37 68
email: cbelei...@units.it
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