On Tue, 2011-10-04 at 08:45 +0000, Ashley Houlden wrote:
> Hi,
<snip />
> #distance matrix of samples using bray curtis
> d= bcdist(mydata, rmzero=FALSE)

In addition, you don't necessarily need ecodist for the bray curtis
distance. vegdist() in vegan will compute this for you.

Not that there is anything wrong with ecodist I hasten to add - just
that you can do this all in vegan if you wanted.

G

> And then using the distance matrix from this to use for adoins? Is this 
> correct.
> 
> With this I have then run Adonis
> 
> results = adonis(d ~ wood, envdata_fit, permutations = 1000)
> 
> and get significant values to see if sig diff in diversity between wood and 
> grass habitat.
> 
> However I have been reading about combining the variables, but there seems to 
> be different ways for example
> 
> results = adonis(d ~ wood+soil, envdata_fit, permutations = 1000)
> 
> so get sig values for Wood and soil
> 
> or
> 
> results = adonis(d ~ wood*soil, envdata_fit, permutations = 1000)
> 
> And I get sig values for wood, soil, and wood soil interaction.
> 
> This seems to make sense, however for both if I put the variable the other 
> way around (soil+wood or soil*wood) I get very different sig values, even 
> accounting for the fact they vary slightly due to the permutations. So whats 
> is going on and why to the the values change so much?
> 
> I was also wondering in Adonis, can you nest treatments, so see effect of 
> soil removing the effect of woodland as you can with anova?
> 
> Another general questions as well, if I have more than two groups in a 
> treatment, say for soil, clay, sand, loam and do the stats, and I get a 
> significant value, what does it actually mean, is it that soil generally has 
> an effect, with each group separate, or there are general differences between 
> soils which may be one group is very different to the other two?
> 
> Many many thanks to anyone who can help me as I have asked people who use R 
> near me and no-one is sure and uses Adonis..
> 
> Ash
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