Hello,
I have RNAseq data, which I am trying to analyze with DESeq. My file (tab
delimited .txt) appears to be correct:
>head(myfile)
VZ_w13 VZ_w14a VZ_w14b VZ_w15a VZ_w15b VZ_w16a
ENSG00000253101 0 0 0 0 0 0
ENSG00000223972 0 0 0 0 0 0...
However, when I try to analyze the data with
>cds <- newCountDataSet(myfile,conds)
I get the following message:
"Error in newCountDataSet(myfile,conds) : The countData is not integer.
The problem, as far as I can tell, is that my data are numerical, not integer,
because when I run
>str(myfile)
'data.frame': 53433 obs. of 14 variables:
$ VZ_w13 : num 0 0 0 0 8 0 0 0 0 0 ...
Does anyone have a way to convert my file from numerical to integer? As you can
see, the data are in fact integers are already, so I'm a bit confused.
Thanks,
Eric
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