Dear List
I have that problem:
this is file : PBS.txt
Time RFU
0.0000 27.3021
0.0800 26.1565
0.1600 25.4836
0.2400 25.2104
0.3200 24.7967
0.4000 24.6310
0.4800 24.3472
0.5600 24.2905
0.6400 24.1394
0.7200 24.0244
0.8000 23.8532
0.8800 23.7908
0.9600 23.6668
1.0400 23.7458
1.1200 23.6787
1.2000 23.5651
1.2800 23.4757
1.3600 23.5172
1.4400 23.4748
1.5200 23.6037
1.6000 23.4516
1.6800 23.5655
1.7600 23.4365
1.8400 23.3553
1.9200 23.5021
2.0000 23.4123
rm(list=ls())
print("ls()")
#carica Dati con file di testo
b <-read.table("PBS.txt", sep="\t", header= TRUE)
print("b")
b
nlmod1 <- nls(RFU ~ A + (B+c*Time)/(1+exp(k*(e-Time))),
data = b,
start = list(A = 1, c = 1, B = 1, k =1, e = 1),
trace = TRUE))
#-------------------------------------------------------------------------------------
13413478 : 1 1 1 1 1
Errore in nls(RFU ~ A + (B + c * Time)/(1 + exp(k * (e - Time))), data = b, :
gradiente singolare
Could you help me? The plot of data I don`t found errors and I don`t know why
sometimes in similar data function and sometimes not.
thanks for the help!
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