Hello,

Try the following.


lmR <- lm(cd$Depth ~ cd$CHAOsep12RNA)
pconfR <- predict(lmR,interval="confidence")
pcR <- pconfR[order(pconfR[, 1]), ]  # Add this line

plot(cd$CHAOsep12RNA,cd$Depth, ylim = rev(range(0:100)), xlab="CHAO", ylab="Depth", pch=15, las=2, main="Sep12-RNA", cex.main=1)
abline(lmR)
matlines(sort(cd$CHAOsep12RNA), pcR[,c("lwr", "upr")], col=1, lty=2) # changed


Hope this helps,

Rui Barradas

Em 17-05-2013 14:03, Ozgul Inceoglu escreveu:
Dear All,

When I plot the values and linear regression line for one data set, it is fine. 
But for another one I see zigzags, when I plot the confidence interval

cd
Depth   CHAOsep12RNA
9,94    804
25,06   1476,833333
40,04   1540,561404
50,11   1575,166667
52,46   349,222222
54,92   1941,5
57,29   1053,507042
60,11   1535,1
70,04   2244,963303
79,97   1954,507042
100,31  2679,140625


plot(cd$CHAOsep12RNA,cd$Depth, ylim = rev(range(0:100)), xlab="CHAO", ylab="Depth", 
pch=15, las=2, main="Sep12-RNA", cex.main=1)
lmR <- lm(cd$Depth~cd$CHAOsep12RNA)
abline(lmR)
pconfR <- predict(lmR,interval="confidence")
matlines(cd$CHAOsep12RNA,pconfR[,c("lwr","upr")], col=1, lty=2)

I also tried

newx <- seq(min(cd$CHAOsep12RNA), max(cd$CHAOsep12RNA), length.out=11)
a <- predict(lmR, newdata=data.frame(CHAO=newx), interval=c("confidence"))
plot(cd$CHAOsep12RNA,cd$Depth, ylim = rev(range(0:100)), xlab="CHAO", ylab="Depth", 
pch=15, las=2, main="Sep12-RNA", cex.main=1)
abline(lmR)
lines(cd$CHAOsep12RNA, a[,2], lty=2)

But I see both cases kind of zigzags. What can it be the reason? thank you!



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