Hi,

I am trying to analyze a data set when nematodes were killed after a
drug administration.

We have counted the number of nematode died and the number of nematode
survival at three time points.

So, there are 100% died in some plot and could be found zero percent
in another. Then, the data set have a lot of zeros.

I have googled and found a lot of information. Moreover, my data isn't
adjust to a normal distribution.

I have transformed it to square root, but, due to zeros, it don't fit
to a normal.

The design is a split-plot. I divided a Petri dish in four parts and
each day we measured one of they.

Here is a sample of the data:

trat    rep     time    killed  living  percent.killed  percent.living
1       1       48      8       6       57.14   42.86
1       2       48      17      15      53.13   46.88
1       3       48      6       4       60.00   40.00
1       1       72      17      15      53.13   46.88
1       2       72      24      33      42.11   57.89
1       3       72      11      0       100.00  0.00
1       1       96      18      28      39.13   60.87
1       2       96      19      6       76.00   24.00
1       3       96      9       10      47.37   52.63
2       1       48      7       2       77.78   22.22
2       2       48      10      4       71.43   28.57
2       3       48      8       2       80.00   20.00
2       1       72      5       2       71.43   28.57
2       2       72      14      13      51.85   48.15
2       3       72      30      1       96.77   3.23
2       1       96      2       6       25.00   75.00
2       2       96      11      15      42.31   57.69
2       3       96      3       2       60.00   40.00
3       1       48      8       8       50.00   50.00
3       2       48      6       7       46.15   53.85
3       3       48      0       2       0.00    100.00
3       1       72      3       3       50.00   50.00
3       2       72      5       1       83.33   16.67
3       3       72      18      10      64.29   35.71
3       1       96      4       0       100.00  0.00
3       2       96      0       0       0.00    0.00
3       3       96      18      19      48.65   51.35

We have counted killed and living because free-living nematode
reproduce very fast, so I need to know the number of living in the
medium.

What you suggest me for analyze this on R? What transformation I could do?

There were a specific package for that?

Have you did something like this?

Thank you very much

-- 
Marcelo Luiz de Laia
Lages - SC - Brazil
Linux user number 487797

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