I never had seen spline() inside some nls()/nlme() function. Are you sure that this fit is possible? Splines makes a lot or successive local polynomial fits, so they need a lot of parameters. I don't think that is possible a good spline fit with only four parameters. In this case you could use loess() or locfit() in locfit library.
Walmes - Brasil. ----- ..oooO .................................................................................................. ..(....)... 0ooo... Walmes Zeviani ...\..(.....(.....)... Master in Statistics and Agricultural Experimentation ....\_)..... )../.... [email protected], Lavras - MG, Brasil ............ (_/............................................................................................ -- View this message in context: http://n4.nabble.com/HELP-on-Non-Linera-Mixed-Effect-model-tp1556953p1557173.html Sent from the R help mailing list archive at Nabble.com. ______________________________________________ [email protected] mailing list https://stat.ethz.ch/mailman/listinfo/r-help PLEASE do read the posting guide http://www.R-project.org/posting-guide.html and provide commented, minimal, self-contained, reproducible code.

