Dear Prof. Bates,

Thank you for your reply. I did actually check the book at pages 89-92, but
I have to say I found it a bit "cryptic", if not downright confusing, for a
genetist like me. Any day of the week. 

To use the example in the book, I cannot see why MACHINE is inner to
WORKER. Or where the Pi = sum of df for the term estimatet at level i... 

To me it's just a two way anova with one random effect + one interaction.
My df correspond to those in the book, but I calculated them in the "bog
standard" way of 2 df for 3 machines, 5 df for 6 workers and 2 * 5 = 10 df
for the interaction...oh well...

I attach the datasest (I did a sample with replace = TRUE, should have
thought of this earlier...). I hope thing would be clearer. It is obvious
that if I consider day a "factor", as I have just one datapoint per day per
line within regime, I end up using all my degrees of freedom:

2 df for 3 regimes
6 df for line within regime
8 df for day (9 days, day as factor)
16 df for regime * day
48 df for line * day
total 80 df, with 0 df remaining for the error. A bit of a problem I
daresay, but I did not collect the data!

BUT I did calculate my anova considering day as a continuous variable,

2 df for 3 regimes
6 df for line within regime
1 df for day (day as a number)
2 df for regime * day
6 df for line * day
total 17 df, with 63 df remaining for the error. 

I still do not get why the interaction term Regime*Day is not tested on the
interaction Line-in-regime*Day... In my analysis LME is clumping Error and
Line-in-regime*Day together, judging by the df I was talking about in my
previuos email. To me it should be just another "bog standard"
situation...But if I were smart enough to be a statistician I would not be
here doing genetics ;)

Regards,
Federico Calboli



Day     Line    Regime  Matings
2       501     es      0.4
4       501     es      0.32989691
9       501     es      0.48484848
11      501     es      0.72727273
16      501     es      0.34042553
18      501     es      0.56470588
25      501     es      0.37509377
30      501     es      0.22222222
32      501     es      0.77777778
2       502     es      0.57142857
4       502     es      1.06666667
9       502     es      0.16
11      502     es      0.4
16      502     es      0.4
18      502     es      0.33333333
25      502     es      0.80808081
30      502     es      0.48
32      502     es      0.25531915
2       503     es      0
4       503     es      0.72727273
9       503     es      0.3
11      503     es      0.77777778
16      503     es      0.34042553
18      503     es      1.06666667
25      503     es      0.32989691
30      503     es      1.24444444
32      503     es      0.19153725
2       fb1     fb      0.72727273
4       fb1     fb      0.42105263
9       fb1     fb      0.32989691
11      fb1     fb      0.32323232
16      fb1     fb      0.37509377
18      fb1     fb      0.51612903
25      fb1     fb      0.4
30      fb1     fb      0.24742268
32      fb1     fb      0.24742268
2       fb2     fb      1.33333333
4       fb2     fb      0.25263158
9       fb2     fb      0.66666667
11      fb2     fb      1.06666667
16      fb2     fb      0.97959184
18      fb2     fb      0.42105263
25      fb2     fb      0.57142857
30      fb2     fb      1.15555556
32      fb2     fb      0.80808081
2       fb3     fb      0.18952106
4       fb3     fb      0.66666667
9       fb3     fb      1.22033898
11      fb3     fb      0.35955056
16      fb3     fb      1.68421053
18      fb3     fb      0.57461174
25      fb3     fb      0.93506493
30      fb3     fb      0.80808081
32      fb3     fb      0.57461174
2       mb1     mb      0.22222222
4       mb1     mb      0.3902439
9       mb1     mb      0.42105263
11      mb1     mb      0.48484848
16      mb1     mb      0.55555556
18      mb1     mb      1.68421053
25      mb1     mb      0.4
30      mb1     mb      0.16
32      mb1     mb      0.09523809
2       mb2     mb      0.77777778
4       mb2     mb      0.68817204
9       mb2     mb      0.25263158
11      mb2     mb      1.33333333
16      mb2     mb      0.5
18      mb2     mb      0.37509377
25      mb2     mb      0.16494845
30      mb2     mb      2.46153846
32      mb2     mb      0.47058824
2       mb3     mb      1.06666667
4       mb3     mb      0.5
9       mb3     mb      0.5
11      mb3     mb      0.38095238
16      mb3     mb      0.26373626
18      mb3     mb      0.87912088
25      mb3     mb      0.55555556
30      mb3     mb      0.74418605
32      mb3     mb      1.33333333

>
>I think your calculation is based on using only within-strata
>information whereas lme uses both within-strata and between-strata
>information for estimates of effects.
>
>The way that we calculate denominator degrees of freedom is described
>on pp. 90-91 of Pinheiro and Bates (2000).  For each term in the
>fixed-effects we determine the innermost level of the random effects
>at which is it changing.  Because Regime is constant for each Line it
>has the fewest degrees of freedom but Day is changing within Line so
>terms in Day have more degrees of freedom.  Is this what you intended?
>
>I must admit I am having difficulty understanding the structure of the
>experiment but it is still Monday morning for me so perhaps that is
>not surprising.


=========================

Federico C.F. Calboli

Department of Biology
University College London
Room 327
Darwin Building
Gower Street
London
WClE 6BT

Tel: (+44) 020 7679 4395 
Fax (+44) 020 7679 7096
[EMAIL PROTECTED]

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