Dear all,

We compared the gene expression level in two conditions: high (H) and low (L), using 
real-time PCR. We had 3 samples of each condition, and we quantified each sample twice 
(i.e. a technical replication). 
Our data looks like: 
H1 H1 H2 H2 H3 H3 L1 L1 L2 L2 L3 L3

We want to test if the level in H vs L is significantly different. I would like to use 
ANOVA  and I guess a nested model with the samples as random  variables should be 
used. How do you do it with R? (assume we don't have much experience with R, nor with 
statistics) 


Thanks in advance, Eran
 



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