Dear Gideon

Unless you are willing to accept uncorrelated measures in time and homogeneity of variance I would go for a linear mixed model instead. You might be surprised on how diffrent can be your results. Try;
?lme

I hope that this helps

Francisco


From: GIDEON WASSERBERG <[EMAIL PROTECTED]>
To: "[EMAIL PROTECTED]" <[EMAIL PROTECTED]>
Subject: [R] 2 way repeated measures ANOVA using R: syntax and reportingquestion
Date: Wed, 26 May 2004 14:03:21 -0500


Dear Friends

I have a technical question about conducting 2 way repeated measures ANOVA analysis using R.

1. Data set: repeated measurement of activity over night (2 hr. intervals)
repeated (within subject)factor: Hours
Between subject: Species, Sex
Dependent variables: specimens

Here is how the data arranged for the analysis:

Subject Replicate       Hour    Sp.     Specimens
1       1              1        a
2       1              1        a
3       1              1        b
4       1              1        b
5       2              1        a
6       2              1        a
7       2              1        b
8       2              1        b
1       1              2        a
2       1              2        a
3       1              2        b
4       1              2        b
5       2              2        a
6       2              2        a
7       2              2        b
8       2              2        b


here's the command I used: summary(aov(Specimens~Species*Sex*Hours+Error(Subject/Hours),data=SP)).

My question is - is that the correct way to do it??

2. Below is the results output:
A. I am not sure I understand what is the meaning of the two error terms I get.
Does the first one show error between subjetcs
and the second - error within subjects?


Error: Subject �
        Df  Sum Sq Mean Sq
Species  1 0.60715 0.60715

Error: Subject:Hours
      Df Sum Sq Mean Sq
Hours  1 158.24  158.24

Error: Within
                    Df Sum Sq Mean Sq F value    Pr(>F)
Species              2  35.19   17.59 34.3017 2.733e-15 ***
Sex                  1  18.98   18.98 37.0092 1.493e-09 ***
Hours                1  40.02   40.02 78.0278 < 2.2e-16 ***
Species:Sex          2   1.29    0.64  1.2568    0.2849
Species:Hours        2   9.95    4.97  9.6993 6.530e-05 ***
Sex:Hours            1   0.66    0.66  1.2778    0.2585
Species:Sex:Hours    2   1.38    0.69  1.3498    0.2596
Residuals         1486 762.20    0.51
---
B. How do I summarize this information for a report?
Is the below suggestion acceptable?
-------------------------------------------------------------------------------

Source                df        SS      MS                       F          P
-----------------------------------------------------------------------------
Species                2        35.19   17.59                   34.3     <0.0001
Sex                    1        18.98   18.98                   37.01     <0.0001
Species:Sex             2       1.29    0.64                   1.2568    0.2849
Subject(Species)        1       0.60715 0.60715
Subject(Hours)         1        158.24  158.24
Hour                    1       40.02     40.02                 78.0278    <0.0001
Species:Hours           2       9.95            4.97             9.6993    <0.0001
Sex:Hours               1       0.66            0.66             1.2778    0.2585
Species:Sex:Hours       2       1.38            0.69             1.3498 0.2596
Residuals            1486       762.20          0.51
---------------------------------------------------------------------------------
Very much obliged for your kind response

Gideon


Gideon Wasserberg (Ph.D.) Wildlife research unit, Department of wildlife ecology, University of Wisconsin 218 Russell labs, 1630 Linden dr., Madison, Wisconsin 53706, USA. Tel.:608 265 2130, Fax: 608 262 6099

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