Papy Gaston wrote:

how I can put two additive effects in random of lme ?

It is best to use the version of lme from the lme4 package to do this.

In that version you can use a formula for random like

  random = ~ 1 | rows + columns

The most general form of the random specification is as a named list where the names correspond to the grouping factors.

  random = list(rows = ~ time, columns = ~ 1)

This only works in the version of lme from the lme4 package. It does not work in the version from the nlme package.

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