Srinivas Iyyer wrote:
Dear group, I am using justRMA in bioconductor to get expression
values. Now I computed fold changes. the fold
changes i get are log2 values.


How can I convert these to log10 values to see them as
actual fold change values.

First, a bit of etiquette. It is considered impolite to post the same question to more than one listserv. Second, although this is not a BioC-specific question, that is probably a better place to ask.


To answer; log10 values will *not* give you actual fold changes. These will be the same as log2, to a multiplicitive constant. You want to convert back to the natural scale e.g., 2^FC, where FC = your fold changes.

Also note that you compute fold change on the log scale by subtraction, not division.

HTH,

Jim



thank you.

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James W. MacDonald
Affymetrix and cDNA Microarray Core
University of Michigan Cancer Center
1500 E. Medical Center Drive
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Ann Arbor MI 48109

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