Quoting [EMAIL PROTECTED]: > Hi everyone, > > I have a question about limmaGUI.I've just started to use the program > for microarray analysis.My problem is after loading data into Limma > (with GAl file and RNA targets file),I'm not able to create an M box > plot and everytime I'm trying to do that, some error appear: > Error in .find.package(pkg): there is no package called 'tkrplot' > Error in try(expr,TRUE): could not find function "tkrplot" > Error in .Tcl.args.objv(...):object "img" not found > I would also like to know if it's necessary to have a spot type file. > I'm using R in windows xp,version 2.3.1 and limmaGUI version 1.8.1 > using limma version 2.7.10. > Could the problem be the version of LimmaGUI I'm using? > I appreciate your help.
The spots file is not necessary. It is used, however, to define individual spots or classes of spots of interests, either to remove from normalisation, as control spots, or just to highlight in different colours on your plots. In windows I recommend you install limmaGUI from: http://bioinf.wehi.edu.au/limmaGUI/ go to section 2.3.1, for a binary that will install R 2.1.1 and limmaGUI with all dependent packages. I hope this helps Jose PS: remember the BioConductor forum, you're more likely to get useful answers about limmaGUI over there. http://www.bioconductor.org/docs/mailList.html -- Dr. Jose I. de las Heras Email: [EMAIL PROTECTED] The Wellcome Trust Centre for Cell Biology Phone: +44 (0)131 6513374 Institute for Cell & Molecular Biology Fax: +44 (0)131 6507360 Swann Building, Mayfield Road University of Edinburgh Edinburgh EH9 3JR UK ______________________________________________ R-help@stat.math.ethz.ch mailing list https://stat.ethz.ch/mailman/listinfo/r-help PLEASE do read the posting guide http://www.R-project.org/posting-guide.html and provide commented, minimal, self-contained, reproducible code.