A difference between two communities within a host could still exist and could make perfect sense, too, when you regard "community" as a random factor. Then "community" may introduce some extra variation (compared to the within-community variation), experimentally seen interesting and important, because the replication of communities makes sure you are not pseudoreplicating. I am not sure however, how to declare the correct df for the random factor in adonis in this case... anybody knows better than I?


Gian Maria Niccolò Benucci wrote:
Maria,


*...Nevertheless you still do not know if your communities are significantly
different between each other, within each host. Now it depends on the
hypothesis you intend to test.*..


I think no sense for "Community" inside "Host"... Couse A and B are from the
same host "Corylus", and C and D are from host "Ostrya".
So the effect between two host tree species is real, but difference between
two community inside the same host (A vs B i.g.) could not be.
That is also confirmed by my diversity indices data I got (see my lastest
post), they show that A and B are alwasy different from C and D, but between
A and B (and for sure also between C and D ) there are no statistical
differences (ANOVA).

I think both "host" and "community" effect and if use them separately I got
:

adonis(sqrtABCD ~ Host, method="bray", data=env.table, permutations=99)

Call: adonis(formula = sqrtABCD ~ Host, data = env.table, permutations =
99,      method = "bray")

                Df SumsOfSqs  MeanSqs  F.Model     R2 Pr(>F)
Host       1.00000   1.64429  1.64429  5.38984 0.1242   0.01 **

Residuals 38.00000  11.59276  0.30507          0.8758
Total     39.00000  13.23705                   1.0000
---
Signif. codes:  0 ‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1 ‘ ’ 1


adonis(sqrtABCD ~ Community, method="bray", data=env.table,
permutations=99)

Call: adonis(formula = sqrtABCD ~ Community, data = env.table, permutations
= 99,      method = "bray")

                Df SumsOfSqs  MeanSqs  F.Model     R2 Pr(>F)
Community  3.00000   2.43264  0.81088  2.70182 0.1838   0.01 **

Residuals 36.00000  10.80441  0.30012          0.8162
Total     39.00000  13.23705                   1.0000
---
Signif. codes:  0 ‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1 ‘ ’ 1

Thank you so much to all want to write any comments on that...

Cheers,


Gian




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